Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠Ⅱ型胶原（Col Ⅱ）酶联免疫检测试剂盒（货号：CSB-E08092r），采用双抗体夹心法定量检测样本中Col Ⅱ的含量，适用于血清、血浆、细胞培养上清液及组织匀浆等多种样本类型。Col Ⅱ是软骨组织的主要结构蛋白，参与维持软骨机械强度和弹性，其表达水平与骨关节炎、软骨损伤修复等病理生理过程密切相关。本试剂盒检测灵敏度高，线性范围为15.6-1000 pg/mL，通过标准曲线可实现精准定量分析，实验操作简便，样本经适当处理后可直接用于检测。该产品可广泛应用于软骨发育、退行性关节疾病机制研究、药物或生物材料对软骨代谢影响的体外评估等科研领域，为探索Col Ⅱ在骨骼系统相关疾病中的作用提供可靠工具，本产品仅限科学研究使用。
别名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：

Col Ⅱ
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-92
1:200	Average %	94
	Range %	90-99
1:400	Average %	92
	Range %	88-96
1:800	Average %	102
	Range %	98-105

回收率：

The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	93-96
EDTA plasma (n=4)	98	89-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.