Mouse Collagen Type Ⅱ,Col Ⅱ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠Ⅱ型胶原(Col Ⅱ)酶联免疫试剂盒（货号：CSB-E08089m），专为定量检测血清、血浆及组织匀浆样本中的Col Ⅱ蛋白设计。Ⅱ型胶原是软骨细胞外基质的主要成分，参与维持软骨组织机械稳定性，其表达水平变化与骨关节炎、软骨损伤修复等研究密切相关。试剂盒基于双抗体夹心法原理，通过预包被特异性捕获抗体，结合样本中的Col Ⅱ蛋白后，利用标记检测抗体进行信号放大，检测灵敏度为1.87 ng/mL，线性范围为1.87-120 ng/mL，适用于微量样本分析（最低样本体积为100 μL）。试剂盒包含预包被96孔板、标准品、生物素化检测抗体及显色底物等核心组分，操作流程可在4.5小时内完成，适用于科研领域中小鼠关节炎模型评估、软骨发育机制研究或退行性关节疾病中Col Ⅱ代谢动态分析，为体外实验提供高特异性的定量检测方案。
别名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：

Col Ⅱ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.87 ng/mL-120 ng/mL
灵敏度：

0.46 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	101
	Range %	94-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	97
	Range %	91-100

回收率：

The recovery of mouse Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-93
EDTA plasma (n=4)	104	95-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.