Rat Advanced glycosylation end product-specific receptor(AGER) ELISA kit

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中文名称：

大鼠晚期糖基化终产物特异性受体(AGER)酶联免疫试剂盒
货号：

CSB-EL001441RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠晚期糖基化终产物特异性受体（AGER）酶联免疫试剂盒（货号：CSB-EL001441RA），采用夹心法原理实现定量检测，适用于分析血清、血浆及组织匀浆样本中AGER的蛋白表达水平，检测灵敏度范围为31.25 pg/mL至2000 pg/mL。AGER作为细胞表面受体，通过与晚期糖基化终产物（AGEs）结合参与炎症反应、氧化应激调控等病理生理过程，其表达异常与糖尿病并发症、神经退行性疾病及动脉硬化等疾病机制密切相关。本试剂盒通过高特异性抗体对目标蛋白进行双位点识别，确保检测结果的准确性和稳定性，实验操作流程标准化，可满足不同浓度样本的检测需求。该产品主要应用于基础研究领域，如糖尿病相关器官损伤的分子机制探索、神经炎症模型的蛋白表达分析，或药物干预下AGER信号通路变化的体外评估，为科研人员提供可靠的实验工具。
别名：

Ager ELISA Kit; Rage ELISA Kit; Advanced glycosylation end product-specific receptor ELISA Kit; Receptor for advanced glycosylation end products ELISA Kit
缩写：

AGER
Uniprot No.：

Q63495
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma,tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	224.423	224.423	225.339	224.377	225.431	224.881
SD	0.034	0.030	0.031	0.040	0.037	0.039
CV(%)	4.433	3.911	4.031	5.216	4.810	5.078

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AGER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

90-94

1:2

Average %

Range %

90-102

1:4

Average %

102

Range %

99-105

1:8

Average %

Range %

80-94

回收率：

The recovery of rat AGER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	97-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

2.703

2.878

2.791

2.615

1000

1.903

1.856

1.880

1.704

500

1.245

1.154

1.200

1.024

250

0.731

0.747

0.739

0.563

125

0.556

0.533

0.545

0.369

62.5

0.402

0.392

0.397

0.221

31.25

0.272

0.275

0.274

0.098

0.173

0.179

0.176

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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最新研究进展：

最新研究发现，AGER基因的多态性可能与各种疾病的发生有关，包括糖尿病、肺炎、慢性阻塞性肺病等。此外，AGER的表达也与肺癌、乳腺癌、胃癌等肿瘤的发生和发展有关。
功能：

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.
亚细胞定位：

Membrane; Single-pass type I membrane protein.
组织特异性：

Endothelial cells and cardiomyocytes.
数据库链接：

STRING: 10116.ENSRNOP00000000508

UniGene: Rn.9829

Rat Advanced glycosylation end product-specific receptor(AGER) ELISA kit

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