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中文名称:大鼠活化素A(ACV-A)酶联免疫试剂盒
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货号:CSB-E04503r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:CUSABIO基于双抗体夹心法原理开发的大鼠活化素A(ACV-A)酶联免疫定量检测试剂盒,适用于血清、血浆及组织匀浆样本中ACV-A蛋白的精准定量分析。活化素A作为转化生长因子β(TGF-β)超家族的重要成员,在调节细胞分化、免疫应答及生殖系统功能中发挥关键作用,其水平变化与炎症反应、代谢调控及组织修复等生理病理过程密切相关。CUSABIO试剂盒采用高特异性配对抗体,可在15.6-1000 pg/mL的检测范围内实现高灵敏度检测,最低检测限为15.6 pg/mL,能够满足低丰度样本的定量需求。实验操作全程耗时约4.5小时,样本仅需50μL即可完成检测,适用于研究ACV-A在生殖生物学、免疫调控机制、代谢性疾病模型中的表达动态,或用于评估药物干预、基因编辑等实验条件下ACV-A的分泌水平变化,为相关领域的分子机制探索提供可靠工具。试剂盒包含预包被酶标板、标准品、检测抗体及全套实验缓冲液,支持科研工作者高效完成实验方案。
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缩写:ACV-A
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:15.6 pg/mL-1000 pg/mL
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灵敏度:3.9 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Signal Transduction
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
250.287
251.377
254.271
255.277
253.524
253.271
SD
0.029
0.030
0.034
0.044
0.042
0.045
CV(%)
3.76
4.61
4.83
6.52
6.46
7.08
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
90
Range %
87-93
1:2
Average %
101
Range %
96-108
1:4
Average %
87
Range %
84-91
1:8
Average %
100
Range %
95-105
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回收率:
The recovery of rat ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
99
95-104
EDTA plasma (n=4)
87
83-90
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.582
2.601
2.592
2.474
500
1.959
1.869
1.914
1.796
250
1.301
1.298
1.300
1.182
125
0.823
0.831
0.827
0.709
62.5
0.498
0.501
0.500
0.382
31.2
0.325
0.336
0.331
0.213
15.6
0.237
0.241
0.239
0.121
0
0.117
0.119
0.118
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数据处理:
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货期:3-5 working days
引用文献
- Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model H Yue,Biomedicine & pharmacotherapy,2022
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