Mouse Activin A,ACV-A ELISA Kit
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中文名称:小鼠活化素A(ACV-A)酶联免疫试剂盒
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货号:CSB-E04502m
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:CUSABIO小鼠活化素A(ACV-A)酶联免疫检测试剂盒(货号CSB-E04502m),采用双抗体夹心法定量分析血清、血浆及组织匀浆样本中ACV-A的浓度,检测范围为15.6-1000 pg/mL,灵敏度为15.6 pg/mL。活化素A作为TGF-β超家族成员,广泛参与细胞增殖分化、胚胎发育及免疫调节等生理过程,其表达水平与炎症反应、组织修复及生殖系统功能密切相关。该试剂盒通过高特异性抗体实现精准检测,适用于小鼠模型在基础研究中的多场景应用,例如探究生殖生物学机制、炎症性疾病模型中的分子调控、组织再生研究或药物干预下ACV-A的动态变化分析。操作流程标准化,可在常规实验室条件下完成样本处理及检测,为科研人员提供稳定可靠的数据支持,助力于发育生物学、免疫学及病理机制等领域的深入探索。
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缩写:ACV-A
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:15.6 pg/mL-1000 pg/mL
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灵敏度:3.9 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Signal Transduction
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
122.000
132.198
122.651
123.043
120.448
120.448
SD
0.055
0.064
0.063
0.071
0.068
0.074
CV(%)
5.56
6.01
6.34
7.12
6.96
7.58
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
86-105
1:2
Average %
96
Range %
91-100
1:4
Average %
93
Range %
90-99
1:8
Average %
96
Range %
89-100
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回收率:
The recovery of mouse ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-99
EDTA plasma (n=4)
97
92-100
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.249
2.268
2.259
2.175
500
2.035
2.055
2.045
1.961
250
1.601
1.586
1.594
1.510
125
0.954
0.972
0.963
0.879
62.5
0.534
0.521
0.528
0.444
31.2
0.272
0.283
0.278
0.194
15.6
0.184
0.190
0.187
0.103
0
0.083
0.085
0.084
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数据处理:
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货期:3-5 working days
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