Rat 4-Hydroxynonenal(HNE)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用竞争法ELISA原理，专用于定量检测大鼠血清或血浆中的4-羟基壬烯酸（HNE），其检测范围为15.6-1000 pg/mL，最低检测限为15.6 pg/mL。HNE是脂质过氧化反应的关键终产物，作为氧化应激的生物标志物，广泛参与炎症反应、动脉粥样硬化及神经退行性疾病等病理过程的研究。本产品通过预包被特异性抗体，结合样本中的HNE与标记物竞争性反应实现定量分析，适用于血清、血浆样本检测，建议样本保存于-20°C并避免反复冻融以保证稳定性。该试剂盒可应用于氧化应激相关机制研究、抗氧化药物干预效果评估，或心血管疾病、代谢综合征等动物模型中脂质过氧化水平的动态监测，为科研人员提供灵敏、可靠的实验工具。
缩写：

HNE
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-92
1:200	Average %	96
	Range %	89-99
1:400	Average %	95
	Range %	91-100
1:800	Average %	93
	Range %	87-98

回收率：

The recovery of rat HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-95
EDTA plasma (n=4)	98	94-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.