Mouse 4-Hydroxynonenal (HNE)ELISA Kit

Mouse 4-Hydroxynonenal (HNE)ELISA Kit

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中文名称：

小鼠4-羟基壬烯酸(HNE)酶联免疫试剂盒
货号：

CSB-E13412m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠4-羟基壬烯酸（HNE）酶联免疫检测试剂盒（货号：CSB-E13412m），基于双抗体夹心法定量分析血清、血浆样本中的HNE水平。HNE是脂质过氧化反应的关键终产物，其含量升高与氧化应激及细胞损伤密切相关，广泛用于评估机体氧化应激状态及相关病理机制研究。该试剂盒检测范围为15.6-1000 pmol/mL，检测下限为15.6 pmol/mL，可精准覆盖生物样本中HNE的典型浓度范围。实验采用预包被特异性抗体的96孔板，通过标准品与样本中HNE的竞争性结合实现定量分析，操作流程标准化，适用于高通量检测。本试剂盒可应用于氧化损伤相关研究领域，如代谢性疾病模型、心血管功能异常或神经退行性病变等科研场景中，通过检测血清或血浆样本的HNE水平，评估氧化应激程度及其在疾病进展中的作用机制，为实验动物模型的病理机制探索提供可靠数据支持。试剂盒包含必要实验组分，需自备酶标仪等基础设备，严格遵循说明书操作可确保结果稳定性。
缩写：

HNE
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

15.6 pmol/mL-1000 pmol/mL
灵敏度：

3.9 pmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	96
	Range %	91-103
1:4	Average %	94
	Range %	90-99
1:8	Average %	94
	Range %	88-98

回收率：

The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-97
EDTA plasma (n=4)	94	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	Corrected
1000	2.608	2.555	2.582	2.392
500	1.939	1.897	1.918	1.728
250	1.227	1.212	1.220	1.030
125	0.821	0.759	0.790	0.600
62.5	0.468	0.450	0.459	0.269
31.2	0.331	0.328	0.330	0.140
15.6	0.278	0.271	0.275	0.085
0	0.192	0.188	0.190

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.