Rabbit soluble P-selectin,sP-selectin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO竞争法ELISA试剂盒，用于定量检测兔源样本中可溶性P选择素（sP-selectin）含量。sP-selectin是一种由血小板和内皮细胞释放的跨膜糖蛋白，在炎症反应、血栓形成及细胞黏附过程中发挥关键作用，其浓度变化常与心血管疾病、免疫调节等研究相关。试剂盒采用生物素标记的特异性抗体与样本中的sP-selectin竞争结合预包被的抗原，通过HRP标记的链霉亲和素系统显色，实现定量分析，检测范围覆盖31.25-2000 pg/mL，适用于血清、血浆、细胞培养上清、尿液、唾液及组织匀浆等多种样本类型。该试剂盒具备高特异性和稳定性，操作流程约需3.5小时，提供预包被96孔板、标准品、检测抗体及终止液等完整组分，可满足基础科研中疾病机制研究、药物作用评估或生物标志物探索等需求，尤其适用于炎症模型、血管功能实验或代谢相关研究的体外样本检测。
缩写：

sP-selectin
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, cell culture supernates, urine, saliva, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	98
	Range %	95-101
1:40	Average %	96
	Range %	93-99
1:80	Average %	87
	Range %	85-90
1:160	Average %	92
	Range %	87-95

回收率：

The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	101	99-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.