Pig soluble P-selectin,sP-Selectin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪可溶性P选择素（sP-Selectin）定量检测试剂盒（货号CSB-E08106p），基于双抗体夹心ELISA原理开发，适用于血清、血浆、细胞培养上清及组织匀浆样本的检测。P选择素作为细胞黏附分子家族成员，在炎症反应和血栓形成中发挥重要作用，其可溶性形式sP-Selectin可作为血管内皮激活和血小板活化的生物标志物。试剂盒检测范围为12.5-800 ng/mL，灵敏度达1.56 ng/mL，采用预包被96孔板设计，标准品及检测试剂即用型分装，包含HRP标记二抗和TMB显色系统，总孵育时间约3小时完成检测流程，操作便捷且重复性良好。该试剂盒适用于科研领域中对猪心血管疾病模型、炎症机制或免疫调控相关研究，可通过血清/血浆样本评估全身性病理状态，结合细胞培养上清分析体外刺激下的分子释放规律，或通过组织匀浆探究局部微环境中sP-Selectin的表达水平，为动物疾病模型构建、药物作用机制探索及生物标志物筛选提供可靠工具。试剂盒组分需-20℃保存，有效期6个月。
缩写：

sP-Selectin
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	95
	Range %	92-98
1:4	Average %	87
	Range %	82-90
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	85	82-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.