Rabbit Estradiol,E2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于检测兔血清或血浆中雌二醇（E2）水平的酶联免疫试剂盒，采用竞争法原理实现定量分析，能够灵敏检测62.5-1000 pg/mL浓度范围的E2含量。雌二醇作为重要的类固醇激素，在兔类生殖系统发育、发情周期调控及卵巢功能维持中发挥核心作用，其浓度变化可反映动物内分泌状态和生殖健康水平。试剂盒包含预包被抗体的96孔板、系列浓度标准品、生物素标记的检测抗体及显色底物，通过生物素-链霉亲和素系统放大信号，结合酶标仪在450 nm波长下进行检测，操作流程标准化且重复性好。适用于科研场景中兔类生殖内分泌机制研究、环境激素干扰评估、药理学实验的激素动态监测，以及畜牧领域繁殖性能相关基础研究。试剂盒组分需在2-8℃避光保存，开封后建议一周内完成检测以确保稳定性，有效期为12个月。本产品仅提供科学研究所需的精准数据支持，不适用于其他用途。
缩写：

E2
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

62.5 pg/mL-1000 pg/mL
灵敏度：

62.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	87
	Range %	84-90
1:4	Average %	87
	Range %	83-91
1:8	Average %	96
	Range %	93-99

回收率：

The recovery of rabbit E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	91-97
EDTA plasma (n=4)	101	98-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.