Fish Estradiol(E2) ELISA kit

Fish Estradiol(E2) ELISA kit

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中文名称：

鱼雌二醇（E2）酶联免疫试剂盒
货号：

CSB-E13017Fh
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO鱼类雌二醇（E2）酶联免疫定量检测试剂盒（货号CSB-E13017Fh），基于竞争法原理设计，适用于血清、血浆样本中E2含量的精确测定。雌二醇（E2）是鱼类体内重要的类固醇激素，主要由卵巢分泌，参与调控生殖系统发育、性腺成熟及次级性征维持等生理过程，其水平变化可反映鱼类的生殖状态及环境内分泌干扰效应。试剂盒检测范围为40 pg/mL至1000 pg/mL，采用预包被抗体的微孔板结构，通过生物素标记抗原与样本中游离E2竞争结合抗体位点，结合辣根过氧化物酶（HRP）标记链霉亲和素系统显色，实现定量分析。该试剂盒具备高灵敏度和特异性，适用于鱼类生理学、内分泌调控机制研究、水产养殖繁殖监测以及水体环境激素污染评估等科研场景。实验操作流程标准化，提供完整的试剂组分（包括标准品、检测抗体、显色底物等），可在常规实验室条件下完成检测，为科研工作者提供稳定可靠的E2定量分析工具。
缩写：

E2
种属：

Fish
样本类型：

serum, plasma
检测范围：

40 pg/ml-1000 pg/ml
灵敏度：

40 pg/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1.826

1.784

1.805

1.308

1.327

1.317

100

0.962

1.028

0.995

200

0.741

0.699

0.720

400

0.529

0.501

0.515

1000

0.336

0.343

0.340

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Reversible Sterilization of Channel Catfish via Overexpression of Glutamic Acid Decarboxylase Gene Z Ye,Animals,2024
Evaluation of the reproductive cycle and gonadal development in the climbing perch, Anabas testudineus (Bloch, 1792) in captivity CV Priyatha,Fisheries,2023
Sequence identification and expression characterization of leptin in the spotted scat, Scatophagus argus WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
Effects of Photoperiod, Water temperature, and Exogenous Hormones on Spawning and Plasma gonadal Steroid in Starry Flounder, Platichthys stellatus BS Hon,/,2021
Three organophosphate flame retardants (OPFRs) reduce sperm quality in Chinese rare minnows (Gobiocypris rarus) R Chen,Environmental Pollution,2020
Possible role of C 60 fullerene in the induction of reproductive toxicity in the freshwater fish, Anabas testudineus (Bloch, 1792) N Sumi,Environmental Science and Pollution Research,2020
Reproductive toxicity and estrogen activity in Japanese medaka (Oryzias latipes) exposed to environmentally relevant concentrations of octocrylene Yan S,Environmental Pollution,2020
Estrogen suppress Bcl2 expression through DNMT1 in Japanese eel ovarian follicle Lin TT, et al,Reproduction,2019
Ctrp9 and adiponectin receptors in Nile tilapia (Oreochromis niloticus): Molecular cloning, tissue distribution and effects on reproductive genes Guokun Yang.et al,General and Comparative Endocrinology,2018
Effect of melatonin on GnIH precursor gene expression in Nile tilapia, Oreochromis niloticus Jung-Hyun Kim.et al,biological rhythm research,2017
2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus) Chen R.et al,Environmental Pollution,2016
Neurokinin B-related Peptide Suppresses the Expression of GnRH I, Kiss2 and tac3 in the Brain of Mature Female Nile tilapia Oreochromis niloticus. Jin YH.et al,Dev Reprod.,2016
The Expression of Leptin, Estrogen Receptors, and Vitellogenin mRNAs in Migrating Female Chum Salmon, Oncorhynchus keta: The Effects of Hypo-osmotic Environmental Changes Young Jae Choi et al, Asian Australas. J. Anim. Sci.,2014
Plasma miRNAs Effectively Distinguish Patients With Pancreatic Cancer From Controls Xu J. et al,Ann Surg,2015
Effect of hypo-osmotic environmental changes on the expression of gonadotropin-releasing hormone, its receptor, and gonadotropin hormone subunit mRNA in adult chum salmon (Oncorhynchus keta) Na Na Kim et al,Marine and Freshwater Behaviour and Physiology,2013

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.