Rabbit Complement 3,C3 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用竞争法原理定量检测兔血清或血浆中的补体蛋白3（C3），其作为补体系统的核心成分，在调理吞噬、炎症反应及免疫复合物清除等先天免疫过程中发挥关键作用。产品货号为CSB-E08668Rb，检测范围为27.3-7000 ng/mL，可精准测定样本中C3浓度，检测原理为样本中的C3与预包被标准品竞争结合特异性抗体，通过酶标二抗显色后，信号强度与C3浓度呈负相关。该试剂盒适用于兔类动物模型中的免疫调控研究，如感染性疾病机制探索、自身免疫病理分析或炎症相关药物开发中的生物标志物检测，为科研人员提供标准化的实验工具。操作流程兼容常规血清/血浆样本预处理，支持96孔板高通量检测，实验数据稳定可靠，可满足基础研究与转化医学中对C3蛋白定量分析的需求。
别名：

AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
缩写：

C3
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

27.3 ng/mL-7000 ng/mL
灵敏度：

27.3 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	91-110
1:2000	Average %	89
	Range %	82-99
1:4000	Average %	95
	Range %	85-99
1:8000	Average %	91
	Range %	87-98

回收率：

The recovery of rabbit C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-97
EDTA plasma (n=4)	97	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.