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  4. Pig Complement 3,C3 ELISA Kit

Pig Complement 3,C3 ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    猪补体蛋白3(C3)酶联免疫试剂盒
  • 货号：
    CSB-E06920p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    猪补体蛋白3（C3）是补体系统的核心成分，通过经典、凝集素和旁路途径参与先天免疫防御，在病原体清除、炎症调节及适应性免疫活化中发挥关键作用。本试剂盒（货号：CSB-E06920p）采用竞争法原理实现定量检测，适用于分析猪血清、血浆及组织匀浆样本中的C3蛋白水平，检测范围为0.625-10 μg/mL，可为科研人员提供精确的浓度测定数据。该试剂盒通过抗原-抗体的特异性结合反应，结合酶标系统显色定量，实验流程标准化且重复性良好。在科研领域，本产品可用于探究猪免疫相关疾病（如细菌/病毒感染、自身免疫性疾病）模型中补体系统的激活机制，评估疫苗或药物干预对补体通路的影响，亦可应用于比较不同遗传背景或饲养条件下猪群体C3表达水平的差异。组织匀浆检测功能还可支持局部炎症或器官特异性免疫反应的研究，例如肠道、肝脏等组织中补体活化与病理进程的关联分析。
  • 别名：
    C3 ELISA Kit; Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA Kit; C3bc); Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein ELISA Kit; ASP ELISA Kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA Kit
  • 缩写：
    C3
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 μg/mL-10 μg/mL
  • 灵敏度：
    0.625 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %96
    Range %91-100
    1:200Average %93
    Range %84-103
    1:400Average %95
    Range %91-101
    1:800Average %93
    Range %83-102
  • 回收率：
    The recovery of pig C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9788-108
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    100.083 0.084 0.084
    50.185 0.176 0.181
    2.50.349 0.352 0.351
    1.250.769 0.776 0.773
    0.6251.285 1.292 1.289
    02.140 2.151 2.146
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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C3 Proteins

靶点详情

  • 功能：
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in post-prandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2.
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: ssc:397072

    STRING: 9823.ENSSSCP00000014399

    UniGene: Ssc.61

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |