Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测兔血清或血浆中Ⅱ型胶原（Col Ⅱ）的酶联免疫试剂盒，采用双抗体夹心法原理，可精准测定6.25-400 ng/mL浓度范围的靶标蛋白。Col Ⅱ是细胞外基质的主要成分，主要分布于软骨组织及玻璃体中，在维持组织结构完整性、调节细胞黏附和分化中起关键作用。本试剂盒包含预包被特异性抗体的微孔板、Col Ⅱ标准品、生物素标记检测抗体、HRP标记链霉亲和素及显色底物，通过标准曲线实现样本中Col Ⅱ的定量分析。适用于科研场景中软骨相关疾病模型研究，如关节炎病理机制探索、软骨损伤修复评估或药物干预效果验证等领域，为组织工程、生物材料开发及退行性疾病研究提供可靠的检测工具。该试剂盒操作流程标准化，灵敏度高，可满足复杂生物样本的检测需求。
别名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
缩写：

Col
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-102
1:2	Average %	107
	Range %	97-111
1:4	Average %	88
	Range %	82-92
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-94
EDTA plasma (n=4)	102	92-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.