Rabbit Collagen Type Ⅳ(COL Ⅳ) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子Ⅳ型胶原(Col Ⅳ)酶联免疫试剂盒（CSB-E14232Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col 含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL，可应用于基础研究领域，如探究肾脏、肝脏等器官纤维化模型中的基底膜代谢异常，分析肿瘤微环境中基质重构机制，或评估血管病变相关疾病模型中Col IV的动态变化。科研人员可通过血清/血浆样本实现纵向监测，或利用组织匀浆进行局部表达水平解析，为组织工程、病理机制及药物干预研究提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)
缩写：

Col
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-91
1:2	Average %	93
	Range %	89-97
1:4	Average %	98
	Range %	95-104
1:8	Average %	86
	Range %	80-94

回收率：

The recovery of rabbit COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	93	87-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.