Pig Stromal cell derived factor 1a,SDF-1a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪基质细胞衍生因子1a（SDF-1a/CXCL12）酶联免疫检测试剂盒，通过夹心法原理实现定量分析，可精准检测血清、血浆、细胞培养上清液、组织匀浆及细胞裂解液等多种样本中的SDF-1a蛋白水平，检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL，满足科研场景下对低浓度样本的检测需求。SDF-1a作为趋化因子CXC亚家族的重要成员，广泛参与细胞定向迁移、免疫调控及组织修复等生物学过程，其表达水平变化与炎症反应、肿瘤转移及干细胞归巢等研究领域密切相关。CUSABIO试剂盒采用高特异性抗体配对，通过预包被捕获抗体和标记检测抗体的双重保障，确保检测结果的高准确性和可重复性，适用于体外培养细胞模型、动物实验组织样本或生物体液中的SDF-1a动态监测，为探索其在心血管疾病、再生医学或免疫微环境调控中的功能机制提供可靠工具。
缩写：

SDF1a/CXCL12
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell Lysates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	92
	Range %	88-96
1:4	Average %	86
	Range %	82-90
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of pig SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	86	82-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.