Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清、血浆、细胞培养上清液及组织匀浆中基质细胞衍生因子1a（SDF-1a/CXCL12）的酶联免疫试剂盒，采用双抗体夹心法原理，灵敏度达0.78 pg/mL，检测范围为0.78-50 pg/mL。SDF-1a作为CXC趋化因子家族成员，在细胞定向迁移、免疫调控及干细胞归巢等生理病理过程中发挥重要作用，其表达水平变化与炎症反应、肿瘤微环境调控和组织修复等研究密切相关。试剂盒包含预包被捕获抗体的96孔板、标准品、生物素标记检测抗体及HRP标记链霉亲和素等核心组分，适用于多种生物样本的直接检测。实验操作中需注意样本预处理：液体样本需离心去除颗粒物，组织样本需匀浆后离心取上清，所有样本应避免反复冻融以维持靶蛋白稳定性。该试剂盒可广泛应用于神经再生、造血调控、免疫细胞趋化性等基础研究领域，为探索SDF-1a/CXCL12在疾病模型中的动态变化提供高特异性检测工具。
缩写：

SDF1a/CXCL12
Uniprot No.：

Q9QZD1
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.78 pg/mL-50 pg/mL
灵敏度：

0.195 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	83-97
1:200	Average %	97
	Range %	91-103
1:400	Average %	84
	Range %	80-88
1:800	Average %	89
	Range %	81-95

回收率：

The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	97	94-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.