Pig Mucin-2,MUC2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪粘蛋白/粘液素2(MUC2)酶联免疫试剂盒（CSB-E15066p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MUC2含量。MUC2是一种分泌型粘蛋白，主要在小肠和结肠的杯状细胞中表达。它在维持肠道稳态中发挥重要作用，其破坏与多种疾病和癌症有关。MUC2的研究涉及其在肠道黏膜屏障损伤中的作用，以及其基因表达调控机制。试剂盒检测范围为7.8 ng/mL-500 ng/mL，该产品适用于猪肠道黏膜屏障功能研究、消化道疾病模型中的黏液分泌机制探索，以及营养干预对肠道健康影响的评估等科研领域，尤其适合通过多类型样本（如肠道组织匀浆与体液样本）对比分析MUC2的动态变化，为猪的肠道生理病理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

MUC2
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig mucin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	87-98
1:8	Average %	92
	Range %	85-95

回收率：

The recovery of pig mucin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-96
EDTA plasma (n=4)	98	95-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.