Mouse Mucin-2,MUC2 ELISA Kit

Mouse Mucin-2,MUC2 ELISA Kit

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中文名称：

小鼠粘蛋白/粘液素2(MUC2)酶联免疫试剂盒
货号：

CSB-E15065m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠粘蛋白/粘液素2（MUC2）酶联免疫试剂盒（货号CSB-E15065m），专用于定量检测血清、血浆、细胞培养上清液及组织匀浆样本中的MUC2蛋白浓度。MUC2是肠道黏液层的主要成分，由杯状细胞分泌形成物理屏障，在调节肠道菌群平衡及黏膜免疫中起关键作用。CUSABIO试剂盒采用双抗体夹心ELISA技术，通过预包被特异性捕获抗体与样本中MUC2结合，再经检测抗体和显色系统实现定量分析，灵敏度达31.25 pg/mL，检测范围为31.25-2000 pg/mL，可精准覆盖低丰度样本的检测需求。试剂盒适用于肠道炎症模型研究、黏液分泌机制探索、黏膜屏障功能评估等科研领域，支持体外实验和动物实验中的动态监测需求。其优化的样本兼容性可处理多种复杂基质，包括含低浓度干扰物质的血浆及经机械破碎的组织匀浆，配套标准品和预混试剂确保操作便捷性和结果一致性，为消化道疾病机制研究及药物开发提供可靠工具。
别名：

Muc2 ELISA Kit; Mucin-2 ELISA Kit; MUC-2 ELISA Kit; Colonic mucin ELISA Kit; MCM ELISA Kit; Secreted gel-forming mucin ELISA Kit; Fragments ELISA Kit
缩写：

MUC2
Uniprot No.：

Q80Z19
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	90
	Range %	83-97
1:40	Average %	95
	Range %	86-107
1:80	Average %	95
	Range %	86-102
1:160	Average %	95
	Range %	88-103

回收率：

The recovery of mouse MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-103
EDTA plasma (n=4)	93	89-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.113	2.024	2.069	1.916
1000	1.626	1.685	1.656	1.503
500	1.105	1.166	1.136	0.983
250	0.643	0.673	0.658	0.505
125	0.458	0.481	0.470	0.317
62.5	0.319	0.337	0.328	0.175
31.25	0.252	0.262	0.257	0.104
0	0.152	0.154	0.153

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Lactobacillus strains reduce the toxic effects of a subchronic exposure to arsenite through drinking water A Domene,Environmental research,2023
Ficolin A knockout alleviates sepsis-induced severe lung injury in mice by restoring gut Akkermansia to inhibit S100A4/STAT3 pathway X Wu,International immunopharmacology,2023
Protective Effects and Mechanism of a Novel Probiotic Strain Ligilactobacillus salivarius YL20 against Cronobacter sakazakii-Induced Necrotizing Enterocolitis In Vitro and In Vivo W Wang,Nutrients,2022
Bacillus subtilis RZ001 improves intestinal integrity and alleviates colitis by inhibiting Notch signaling pathway and activating ATOH-1 Li Y,Pathogens and Disease,2020
Dendrobium huoshanense polysaccharide regionally regulates intestinal mucosal barrier function and intestinal microbiota in mice Song-ZiXie, et al,Carbohydrate Polymers,2018

功能：

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
亚细胞定位：

Secreted. Note=In the intestine, secreted into the inner and outer mucus layers.
组织特异性：

Highly expressed in goblet cells of the colon with lower levels in the small intestine and no expression in the stomach (at protein level).
数据库链接：

STRING: 10090.ENSMUSP00000026590

UniGene: Mm.2041

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.