Pig Immunoglobulin G(IgG) ELISA Kit

Pig Immunoglobulin G(IgG) ELISA Kit

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中文名称：

猪免疫球蛋白G(IgG)酶联免疫试剂盒
货号：

CSB-E06804p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专用于科研领域的猪免疫球蛋白G（IgG）酶联免疫检测试剂盒（货号：CSB-E06804p），基于竞争法原理实现定量分析，适用于检测血清、血浆、细胞培养上清及组织匀浆样本中IgG的表达水平。猪IgG作为体液免疫的核心抗体，在病原体中和、免疫调节及炎症反应中发挥重要作用，其定量检测可为免疫机制研究、疫苗效果评估或疾病模型构建提供关键数据。该试剂盒检测范围覆盖0.586至150 μg/mL的宽线性区间，可精准识别低浓度样本，满足不同实验场景的灵敏度需求。样本需经离心等预处理后按标准流程操作，试剂盒包含预包被板、标准品、检测抗体及显色系统等核心组分，支持实验室高效完成猪IgG的动态监测，尤其适用于畜牧病原体感染研究、生物制品质量控制及免疫应答动态分析等科研方向，为动物免疫学研究提供可靠工具。
别名：

N/A
缩写：

IgG
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.586 μg/mL-150 μg/mL
灵敏度：

0.467 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	90
	Range %	85-96
1:400	Average %	95
	Range %	91-107
1:800	Average %	93
	Range %	86-99
1:1600	Average %	91
	Range %	88-97

回收率：

The recovery of pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	94	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
150	0.254	0.251	0.253
37.5	0.555	0.517	0.536
9.38	0.755	0.721	0.738
2.34	0.999	0.981	0.990
0.58	1.169	1.113	1.141
0	1.481	1.465	1.473

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Maternal consumption of glycerol monolaurate optimizes milk fatty acid profile and enhances piglet gut health in association with G protein-coupled receptor 84 (GPR84) activation L Xiong, Z Zhang, S Dong, T Lin, X Yue, F Chen,Animal Nutrition,2024
Knockdown of swine leukocyte antigen expression in porcine lung transplants enables graft survival without immunosuppression C Figueiredo,Science translational medicine,2024
Effects of inactivated Lactobacillus rhamnosus on growth performance, serum indicators, and colonic microbiota and metabolism of weaned piglets Z Shu, J Zhang, Q Zhou, Y Peng, Y Huang,BMC Veterinary Research,2024
Dietary taurine supplementation counteracts deoxynivalenol-induced liver injury via alleviating oxidative stress, mitochondrial dysfunction, apoptosis, and inflammation in piglets X Ji,Ecotoxicology and environmental safety,2023
Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets Z Yang,Animal Nutrition,2022
Piglets' behaviour and performance in relation to sow characteristics EM Baxter,animal,2022
Supplementation alpha glycerol monolaurate during late gestation and lactation enhanced sow performance, ameliorated milk composition, and improved growth of suckling piglets L Li,researchsquare,2022
Comparative Effects of Compound Enzyme and Antibiotics on Growth Performance, Nutrient Digestibility, Blood Biochemical Index, and Intestinal Health in Weaned Pigs Zhiqing Li,Frontiers in Microbiology,2021
Effect of deep-sea mineral water on growth performance, water intake, blood characteristics and serum immunoglobulins in the growing-finishing pigs SH Lee,J Anim Sci Technol,2021
Effects of Increasing Doses of Lactobacillus Pre-Fermented Rapeseed Product with or without Inclusion of Macroalgae Product on Weaner Piglet Performance and … GD Satessa,animals,2020
Differences in Gut Microbial and Serum Biochemical Indices Between Sows With Different Productive Capacities During Perinatal Period Shao Y,Front Microbiol,2020
Impact of Dietary Supplementation of Lactic Acid Bacteria Fermented Rapeseed with or without Macroalgae on Performance and Health of Piglets Following Omission … Satessa GD,Animals (Basel),2020
Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,2018
Increasing selenium supply for heat-stressed or actively cooled sows improves piglet preweaning survival, colostrum and milk composition, as well as maternal selenium, antioxidant status and immunoglobulin transfer JunChen, et al,Journal of trace elements in medicine and biology,2018
Effects of dietary supplementation with essential oils and organic acids on the growth performance, immune system, faecal volatile fatty acids and microflora community in weaned piglets Yang CM, et al,Journal of Animal Science,2018
Effect of dietary copper source (inorganic vs. chelated) on immune response, mineral status, and fecal mineral excretion in nursery piglets Peng Liao.et al,Food and Agricultural Immunology ,2017
Effects of dietary supplementation with cupreous N-carbamylglutamate (NCG) chelate and copper sulfate on growth performance, serum biochemical profile and immune response, tissue mineral levels and fecal excretion of mineral in weaning piglets Peng Liao.et al,Food and Agricultural Immunology ,2017
Oral administration of N-carbamylglutamate might improve growth performance and intestinal function of suckling piglets Xiangfang Zeng.et al,Livestock Science,2015
Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012
Dietary N-Carbamylglutamate Supplementation Boosts Intestinal Mucosal Immunity in Escherichia coli Challenged Piglets Fengrui Zhang et al,PLoS One,2013

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平均分:

5.0分 - 1 个评价

样品类型：血清

样品信息：其他猪

稀释比：1:100

产品评价：标曲准确，样本数值在标曲范围之内，符合预期结果。

By 谢老师

最新研究进展：

IgG是一种免疫球蛋白，主要存在于血液和淋巴组织中。近年来的研究发现，IgG可以通过特异性结合抗原来识别和中和病原体，从而在机体的免疫防御中起着关键作用。除了作为一种免疫分子，IgG还被广泛用于治疗免疫相关疾病。最近的研究表明，IgG具有对自身免疫性疾病、恶性肿瘤和传染性疾病等多种疾病的治疗潜力。此外，研究人员还在探索IgG的新应用，例如在制备疫苗和免疫治疗中的应用等。

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.