Bovine Immunoglobulin G,IgG ELISA Kit

Bovine Immunoglobulin G,IgG ELISA Kit

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中文名称：

牛免疫球蛋白G(IgG)酶联免疫试剂盒
货号：

CSB-E12015B
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO牛免疫球蛋白G(IgG)酶联免疫试剂盒（货号CSB-E12015B），采用竞争法原理定量检测血清、血浆样本中的牛IgG含量，检测范围为78-5000 ng/ml。牛IgG是反刍动物体液免疫的核心抗体成分，在病原体中和、调理吞噬等免疫防御中发挥重要作用，其水平监测对动物疫病研究、疫苗免疫效果评估及乳制品抗体残留分析具有科研价值。试剂盒采用预包被抗体的固相载体，实验时样本中的牛IgG与酶标记物竞争结合抗体，通过显色反应强度实现定量分析。该检测体系适用于科研领域中对牛源生物样本的免疫指标分析，如牧场健康管理中的群体免疫状态跟踪、乳制品加工过程中免疫活性成分的质控研究，以及生物制剂开发中抗体稳定性的体外评估等，为畜牧兽医研究、食品安全检测及生物医药研发提供可靠的数据支持。本产品仅用于科学研究，实验需严格遵循说明书操作规范以确保结果准确性。
缩写：

IgG
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

78 ng/ml-5000ng/ml
灵敏度：

78 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	85-92
1:200	Average %	97
	Range %	92-101
1:400	Average %	91
	Range %	87-94
1:800	Average %	95
	Range %	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

5000

0.099

0.101

0.100

2500

0.179

0.171

0.175

1250

0.291

0.286

0.289

625

0.500

0.482

0.491

312

0.936

0.917

0.927

156

1.281

1.223

1.252

1.967

1.986

1.977

2.673

2.758

2.716

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Early-life Clostridium butyricum supplementation improved rumen development and immune by promoting the maturation of intestinal microbiota Y Zhu, Z Li, Y Yang, T Zhan, D Bu, L Ma,Journal of Agriculture and Food Research,2024
Immunity, rumen metagenomics, ruminal variables, and growth performance of calves fed milk with sage (Salvia officinalis) essential oil K Kara,Tropical animal health and production,2023
Effects of Lonicera japonica Extract with Different Contents of Chlorogenic Acid on Lactation Performance, Serum Parameters, and Rumen Fermentation in Heat-Stressed Holstein High-Yielding Dairy Cows F Ma,Animals,2024
The influence of passive colostrum transfer on humoral immunity to selected antigens of Mannheimia haemolytica in calves A Wernicki,Acta Veterinaria Brno,2023
Phyllanthus emblica (Amla) Fruit Powder as a Supplement to Improve Preweaning Dairy Calves’ Health: Effect on Antioxidant Capacity, Immune Response, and Gut Bacterial Diversity M Nguse,Biology,2022
Body Condition, Energy Balance and Immune Status of Periparturient Murrah Buffaloes (Bubalus bubalis) Supplemented with Inorganic Chromium Rijusmita Sarma Deka,Biological trace element research,2016
Therapeutic and Prophylactic Effect of the Experimental Bacteriophage Treatment to Control Diarrhea Caused by E. coli in Newborn Calves MMM Alomari,ACS Infectious Diseases,2021
Supplemental chromium in cold-stressed buffalo calves (Bubalus bubalis): effects on growth performance, nutrient utilization and cell mediated and humoral immune response Muneendra Kumar.et al,VETERINARSKI ARHIV ,2017
Dietary Inorganic Chromium in Summer-Exposed Buffalo Calves (Bubalus bubalis): Effects on Biomarkers of Heat Stress, Immune Status, and Endocrine Variables Kumar M et al,Biol Trace Elem Res,2015
Improvements of immune status, intestinal integrity and gain performance in the early-weaned calves parenterally supplemented with l-alanyl-l-glutamine dipeptide /,Veterinary Immunology and Immunopathology,2012

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最新研究进展：

IgG是一种免疫球蛋白，广泛存在于人体中，具有识别、结合和清除病原体的作用。最新的研究表明，IgG不仅具有免疫功能，还参与调节神经系统的生理和病理过程。特别是，IgG的Fc区域在神经发育、神经保护和修复等方面发挥重要作用。此外，IgG还被发现与心血管疾病、肿瘤和炎症等疾病的发生和发展有关。

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.