Mouse α-Melanocyte Stimulating Hormone(α-MSH) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠α-黑色素细胞刺激素(α-MSH)酶联免疫试剂盒（CSB-E15876m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的α-MSH含量。α - MSH即α - 促黑素细胞激素，它是一种内源性神经肽。背景上，其在体内多种生理调节中起重要作用。研究机制方面，它能与相应受体结合，参与调节黑素生成、能量代谢、免疫反应及炎症调控等过程，是当前生物学和医学领域的研究热点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于体外实验模型中α-MSH分泌水平的动态监测，例如评估药物干预对神经内分泌通路的影响、炎症模型中肽类激素变化分析，或黑色素细胞功能相关的基础研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

alpha-melanocyte stimulating hormone, alpha-MSH
缩写：

α-MSH
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-95
1:2	Average %	92
	Range %	88-99
1:4	Average %	94
	Range %	90-99
1:8	Average %	93
	Range %	87-98

回收率：

The recovery of mouse α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	95	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.