Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人α-黑色素细胞刺激素(α-MSH)酶联免疫试剂盒（CSB-E09658h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的α-MSH含量。α - MSH即α - 促黑素细胞激素，是一种内源性神经肽。它在调节皮肤色素沉着、能量代谢、炎症反应等方面发挥重要作用。研究机制主要涉及与黑素皮质素受体结合，激活下游信号通路，进而对多种生理功能产生影响。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品广泛应用于基础科研领域，如探究α-MSH在代谢综合征动物模型中的表达特征、评估体外细胞刺激实验的激素分泌水平，或解析皮肤黑色素生成调控机制等研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

alpha-melanocyte stimulating hormone, alpha-MSH
缩写：

α-MSH
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Biochemicals
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-104
1:2	Average %	95
	Range %	91-104
1:4	Average %	89
	Range %	85-93
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of human α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	94	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 个评价

样品类型：细胞培养上清液

样品信息：人

稀释比：没有稀释

产品评价：我用的是人源α-MSH试剂盒。本次实验目的：探究UVB对角质细胞分泌α-MSH的影响及多肽样品的作用。实验样品：UVB照射后，细胞培养箱中孵育24h，收取细胞分泌上清液。实验组OD值是0.15-0.31。以上为近期所用后的反馈，请查收！

By 雷老师