Mouse tumor necrosis factor α (TNF-α) antibody ELISA Kit

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中文名称：

小鼠肿瘤坏死因子α(TNF-α)抗体酶联免疫试剂盒
货号：

CSB-EQ023955MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠肿瘤坏死因子α（TNF-α）抗体酶联免疫试剂盒（货号：CSB-EQ023955MO），采用双抗体夹心法定量检测血清或血浆样本中的TNF-α含量。TNF-α是由巨噬细胞、T细胞等分泌的重要炎症因子，在免疫应答、细胞凋亡及炎症性疾病研究中具有关键作用。试剂盒灵敏度达0.156 ng/mL，检测范围为0.156-10 ng/mL，适用于低丰度样本分析。实验操作基于预包被特异性抗体的96孔板，通过与样本中的TNF-α结合形成抗体-抗原-酶标抗体复合物，经显色反应实现定量检测。试剂盒包含标准品、检测抗体、显色底物等核心组分，支持血清或血浆样本直接检测（需离心去除纤维蛋白或细胞碎片），适用于炎症机制研究、免疫治疗药物体外效果评估、感染性疾病模型分析等科研场景。该检测体系经过严格验证，可在3.5小时内完成实验流程，为研究感染性休克、类风湿性关节炎等TNF-α相关疾病的分子机制提供可靠工具。
别名：

antibody for DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
缩写：

TNF-α Ab
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-α antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	97
	Range %	90-105
1:4	Average %	87-96
	Range %	82-96
1:8	Average %	93
	Range %	88-100

回收率：

The recovery of mouse TNF-α antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	92	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.713	2.752	2.733	2.580
5	2.212	2.349	2.281	2.128
2.5	1.514	1.623	1.569	1.416
1.25	0.849	0.864	0.857	0.704
0.625	0.502	0.525	0.514	0.361
0.312	0.313	0.316	0.315	0.162
0.156	0.257	0.270	0.264	0.111
0	0.148	0.157	0.153