Human Tumor necrosis factor α (TNF-α) antibody ELISA kit

中文名称：

人肿瘤坏死因子α(TNF-α)抗体酶联免疫试剂盒
货号：

CSB-EQ023955HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人肿瘤坏死因子α（TNF-α）抗体酶联免疫试剂盒（货号：CSB-EQ023955HU），基于间接法原理实现定性检测，适用于科研领域对血清、血浆样本中TNF-α抗体的分析。TNF-α作为关键炎症因子，广泛参与免疫调节、细胞凋亡及炎症反应，其抗体水平的检测可为自身免疫性疾病、感染性疾病及肿瘤微环境研究提供重要依据。试剂盒采用预包被TNF-α抗原捕获样本中特异性抗体，通过酶标二抗显色实现目标信号的可视化判读，具备高特异性和重复性。实验操作全程配备标准化阴阳性对照，确保检测体系可靠性。本产品适用于体外研究场景，包括但不限于疾病机制探索、药物靶点验证或生物标志物筛选等科研项目，实验时需按照标准流程进行样本预处理，建议使用新鲜分离或-20℃保存的血清/血浆样本以保证检测稳定性，具体检测范围需通过实验验证或联系技术支持获取。
别名：

antibody for DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
缩写：

TNF-α Ab
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

Request Information
灵敏度：

Request Information
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

qualitative
测定方法：

Indirect

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

标准曲线：

Test parameter
specification
test result
Positive control
>0.8
1.742
Negative control
<0.2
0.156
Positive rate
10，Positive
100%
Negative rate
10，Negative
100%
货期：

3-5 working days

引用文献

Notch Signaling Regulates the Function and Phenotype of Dendritic Cells in Helicobacter pylori Infection Q Liu,Microorganisms,2023
LncRNA RP11-86H7.1 promotes airway inflammation induced by TRAPM2.5 by acting as a ceRNA of miRNA-9-5p to regulate NFKB1 in HBECS J Zhao,Scientific Reports,2020
Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells Chen X.et al,Cell Physiol Biochem,2018
NR4A1 Promotes Diabetic Nephropathy by Activating Mff-Mediated Mitochondrial Fission and Suppressing Parkin-Mediated Mitophagy Sheng J.et al,Cell Physiol Biochem,2018
Notch signaling pathway suppresses CD8+ T cells activity in patients with lung adenocarcinoma Shuo Li, .et al,International Immunopharmacology,2018
Additive Benefits of Twice Forest Bathing Trips in Elderly Patients with Chronic Heart Failure Gen Xiang MAO.et al,Biomedical and Environmental Sciences,2018
Associations of Salivary BPIFA1 Protein in Chronic Periodontitis Patients with Type 2 Diabetes Mellitus Yue Guo.et al,International Journal of Endocrinology,2017
Synovial fluid concentrations of cold-inducible RNA-binding protein are associated with severity in knee osteoarthritis Yu L.et al,Clin Chim Acta.,2017
Increased neutralization capacity of TNF-a in sera of relapsing remitting multiple sclerosis patients is not related to soluble TNF-a receptors or anti-TNF-aautoantibody levels Karin Mausner-Fainberg. et al,Journal of Neuroimmunology,2015

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Human Tumor necrosis factor α (TNF-α) antibody ELISA kit

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Test parameter	specification	test result
Positive control	>0.8	1.742
Negative control	<0.2	0.156
Positive rate	10，Positive	100%
Negative rate	10，Negative	100%