Mouse telomerase,TE ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠端粒酶（TE）酶联免疫检测试剂盒（货号：CSB-E08022m），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中TE蛋白含量的精准分析。端粒酶作为维持端粒长度的关键逆转录酶，在细胞增殖、衰老调控及肿瘤发生等生命过程中发挥重要作用，是细胞生物学和癌症研究的重要靶点。本试剂盒检测灵敏度高，线性范围为0.156 ng/mL至10 ng/mL，可满足不同浓度样本的检测需求，操作流程标准化且重复性好。产品广泛应用于科研领域，包括但不限于端粒酶活性相关的基础机制研究、抗衰老药物筛选、肿瘤细胞增殖模型构建以及再生医学中的干细胞功能评估等实验场景，为探索TE在疾病发生发展中的作用提供可靠工具。试剂盒组分包含预包被抗体、检测抗体及配套缓冲液，支持常规实验室操作环境下的稳定检测。
别名：

N/A
缩写：

TE
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	82-91
1:10	Average %	104
	Range %	100-108
1:20	Average %	99
	Range %	96-105
1:40	Average %	91
	Range %	87-99

回收率：

The recovery of mouse TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	88	83-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.