Human telomerase,TE ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人端粒酶（TE）酶联免疫检测试剂盒（货号：CSB-E08021h），采用夹心法原理实现定量检测，适用于检测血清、细胞培养上清、尿液及组织匀浆样本中0.312-20 ng/mL浓度范围的端粒酶水平。端粒酶作为维持端粒长度的关键逆转录酶，其活性与细胞增殖、衰老及肿瘤发生密切相关，是癌症研究和细胞生物学领域的重要靶标。本试剂盒灵敏度达0.078 ng/mL，实验流程约需3小时完成，包含预包被96孔板、重组蛋白标准品及辣根过氧化物酶标记的多克隆检测抗体，可在-20℃稳定保存6个月。科研人员可通过该试剂盒分析疾病模型动物或体外培养细胞中的端粒酶表达动态，探索肿瘤发生机制、评估抗衰老药物对端粒酶活性的调控作用，或利用尿液样本开展无创性研究。其宽泛的样本兼容性为组织水平研究、细胞实验数据验证及生物标志物筛选提供了可靠工具，适用于基础医学、药理学及分子生物学领域的定量分析需求。
别名：

N/A
缩写：

TE
种属：

Homo sapiens (Human)
样本类型：

serum, cell culture supernates, urine, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-96
1:2	Average %	99
	Range %	91-105
1:4	Average %	94
	Range %	92-98
1:8	Average %	89
	Range %	86-100

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.