Mouse Kallikrein 1-related peptidase b3,Klk3 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠激肽释放酶1相关肽酶b3（KLK3）酶联免疫试剂盒（货号：CSB-E08276m），采用双抗体夹心法定量检测样本中KLK3的浓度。KLK3属于激肽释放酶家族成员，参与调控细胞外基质代谢及信号转导通路，在生殖系统、肿瘤微环境等生理病理过程中具有潜在研究价值。试剂盒检测范围覆盖0.312 ng/mL至20 ng/mL，适用于血清、血浆及组织匀浆样本的定量分析，灵敏度达0.078 ng/mL，实验全程约需4.5小时，包含预包被捕获抗体、生物素标记检测抗体及标准品等核心组分。该产品可广泛应用于小鼠模型的基础研究领域，例如通过检测血清或组织中的KLK3表达水平，探索其在前列腺疾病、肿瘤进展或炎症反应中的作用机制，亦可用于药物干预实验中的生物标志物动态监测，为科研人员提供稳定、高效的体外检测工具。
别名：

N/A
缩写：

KLK3
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-100
1:2	Average %	93
	Range %	88-97
1:4	Average %	89
	Range %	84-95
1:8	Average %	104
	Range %	100-108

回收率：

The recovery of mouse KLK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	90	85-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.