Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

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中文名称：

小鼠白介素18结合蛋白(IL-18BP)酶联免疫试剂盒
货号：

CSB-E17797m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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本试剂盒专为定量检测小鼠血清、血浆及细胞培养上清液中的白介素18结合蛋白（IL-18BP）设计，该蛋白作为IL-18的天然拮抗剂，通过特异性结合抑制IL-18介导的炎症反应，在免疫调节和炎症相关疾病机制研究中具有重要意义。采用双抗体夹心ELISA技术原理，检测灵敏度最低可达0.156 ng/mL，线性范围覆盖0.156-10 ng/mL，适用于微量样本中IL-18BP的精准定量分析。试剂盒包含预包被捕获抗体、生物素标记检测抗体及标准品等核心组分，操作流程经过优化，可在4.5小时内完成检测。科研人员可将其应用于炎症性疾病动物模型的机制研究、药物干预效果评估或体外细胞实验中IL-18/IL-18BP信号通路的动态监测，特别适用于探索类风湿性关节炎、炎症性肠病等病理过程中IL-18通路的调控机制。该试剂盒严格验证了批次间稳定性与样本兼容性，支持血清/血浆样本直接检测，无需特殊前处理，为科研实验提供高效可靠的工具。
别名：

Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit
缩写：

IL-18BP
Uniprot No.：

Q9Z0M9
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-100
1:2	Average %	84
	Range %	80-88
1:4	Average %	104
	Range %	100-107
1:8	Average %	91
	Range %	87-95

回收率：

The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	89	84-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.729	2.799	2.764	2.592
5	2.302	2.342	2.322	2.150
2.5	1.513	1.587	1.550	1.378
1.25	0.928	0.965	0.947	0.775
0.625	0.548	0.539	0.544	0.372
0.312	0.381	0.375	0.378	0.206
0.156	0.278	0.274	0.276	0.104
0	0.171	0.173	0.172

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

A lncRNA from an inflammatory bowel disease risk locus maintains intestinal host-commensal homeostasis H Ma,Cell research,2023

平均分:

5.0分 - 2 个评价

样品类型：组织匀浆液小鼠结肠

样品信息：小鼠

稀释比：其他稀释2倍

产品评价：我用了货号为CSB-E17797m的Mouse Interleukin-18 Binding Prorein (IL-18BP)ELISA Kit检测了DSS诱导结肠炎之后小鼠结肠组织匀浆中的IL-18BP蛋白，OD值为2左右，该试剂盒效果不错，反应灵敏，达到了预期实验的目的

By 胡老师

样品类型：血清

样品信息：小鼠

稀释比：没有稀释

产品评价：我用CSB-E17797m检测正常小鼠和造模狼疮小鼠的血清，未稀释样品进行检测。标准品OD450值最高为1.99，最低为0.02，标准曲线R平方大于0.99。小鼠血清OD450值范围约为0.3-1.3左右, 对应浓度为1.5-7ng/mL, 符合我的预期浓度范围。

By 唐老师

功能：

Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
基因功能参考文献：
1. IL-18BP ameliorates cardiac ischemia/reperfusion injury in part through suppression of Th17 differentiation. PMID: 26340315
2. Data show that IL-18bp acts as a sensor for IFN-gamma and can regulate both Th1 and Th17 responses in the CNS. PMID: 20644165
3. Administratioan of murine IL-18BP at the time of the booster injection of bovine CII reduces the progressive severity of collagen-induced arthritis; both clinical disease activity and histological scores are decreased by 50%. PMID: 12574381
4. functional polarization of Ag-specific CD4(+) Th2 cells entering the CNS during the accelerating phase of experimental autoimmune encephalomyelitis is flexible and dependent on IL-18, which is overturned by IL-18 binding protein PMID: 15778395
5. IL-18 and IL-18BP may have roles in testicular development, and also in the regulation of testicular functions under physiological conditions PMID: 18299271
6. A single intravenous dose of recombinant mouse IL18BP decreased neointimal hyperplasia and improved arterial L/A ratios in an atherosclerotic balloon-injury animal model. PMID: 18455646
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亚细胞定位：

Secreted.
数据库链接：

KEGG: mmu:16068

STRING: 10090.ENSMUSP00000091685

UniGene: Mm.422756

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.