Mouse complement fragment 3b (C3b) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠补体片断3b(C3b)酶联免疫试剂盒（CSB-EQ027757MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C3b含量。C3b是补体系统中C3被裂解后产生的重要片段。其背景在于补体系统是机体免疫防御的关键部分。研究机制上，C3b参与免疫调理、补体激活经典和旁路途径等，通过与多种细胞表面受体结合，发挥免疫监视、清除病原体等功能。试剂盒检测范围为0.235 ng/mL-15 ng/mL，适用于科研场景下评估补体系统激活程度，如炎症模型动物体液样本分析、免疫相关疾病机制研究中C3b动态监测，或通过组织匀浆检测探究局部微环境补体活性变化，为免疫调控研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

C3b
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.235 ng/mL-15 ng/mL
灵敏度：

0.059 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	103
	Range %	99-105
1:8	Average %	99
	Range %	95-101

回收率：

The recovery of mouse C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-97
EDTA plasma (n=4)	89	86-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.