Human Complement fragment 3b,C3b ELISA Kit

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中文名称：

人补体片断3b(C3b)酶联免疫试剂盒
货号：

CSB-E09303h
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO人补体片断3b（C3b）酶联免疫试剂盒（货号：CSB-E09303h），采用双抗体夹心法原理实现定量检测，可精准分析血清、尿液、脑脊液（CSF）及唾液等多种生物样本中的C3b蛋白浓度。C3b是补体系统活化过程中的关键裂解产物，通过调理作用促进病原体清除，并参与膜攻击复合物形成，其水平变化与感染、自身免疫性疾病及炎症反应密切相关。该试剂盒检测范围为3.12-200 ng/mL，最低检测限为3.12 ng/mL，通过预包被特异性抗体捕获目标蛋白，结合HRP标记检测抗体实现信号放大，配套提供标准品及显色底物，操作流程标准化。本产品适用于基础医学研究中对补体系统活性的动态监测，例如探究感染性疾病、神经炎症或慢性炎症模型中C3b的生物学功能，也可用于评估药物或生物制剂对补体活化途径的调控作用。试剂盒组分需按说明书保存（短期4°C，长期-20°C），实验前需平衡至室温，推荐使用新鲜采集样本以避免反复冻融对检测结果的影响。
缩写：

C3b
种属：

Homo sapiens (Human)
样本类型：

serum, urine, cerebrospinal fluid (CSF), saliva
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-92

1:2

Average %

Range %

92-103

1:4

Average %

Range %

93-103

1:8

Average %

Range %

89-98

回收率：

The recovery of human C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	97	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.115	2.003	2.059	1.932
100	1.748	1.736	1.742	1.615
50	1.245	1.266	1.256	1.129
25	0.756	0.782	0.769	0.642
12.5	0.568	0.572	0.570	0.443
6.25	0.333	0.342	0.338	0.211
3.12	0.199	0.187	0.193	0.066
0	0.128	0.126	0.127

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
Predictive value of soluble CD59 for poor 28-day neurological prognosis and all-cause mortality in patients after cardiopulmonary resuscitation: a prospective observatory study L Wang,Journal of intensive care,2023
Intrathecal complement activation by the classical pathway in tick-borne encephalitis Veje M, et al,Journal of neurovirology,2019
Diminished expression of β2-GPI is associated with a reduced ability to mitigate complement activation in anti-GPIIb/IIIa-mediated immune thrombocytopenia Zhu X.et al,Ann Hematol,2017
Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis. Eriksson CE.et al,J Neuroimmunol.,2016

Human Complement fragment 3b,C3b ELISA Kit

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