Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

Mouse angiotensin Ⅱ(ANG-Ⅱ) ELISA Kit

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中文名称：

小鼠血管紧张素Ⅱ(ANG-Ⅱ)酶联免疫试剂盒
货号：

CSB-E04495m
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠血管紧张素Ⅱ（ANG-Ⅱ）酶联免疫检测试剂盒（货号：CSB-E04495m），采用竞争法原理实现定量分析，适用于血清、血浆样本中ANG-Ⅱ含量的精确检测。ANG-Ⅱ作为肾素-血管紧张素系统（RAS）的核心效应分子，在调节血压、体液平衡及心血管功能中发挥关键作用，其水平变化与高血压、心脏肥大等病理过程密切相关。本试剂盒检测范围为15.6-1000 pg/ml，通过预包被特异性抗体的酶标板捕获样本中的ANG-Ⅱ分子，竞争性结合标记物后经显色反应测定吸光度值，目标物浓度与信号强度呈负相关，操作流程包含样本处理、孵育、洗涤和读数等标准化步骤，可在4-6小时内完成检测。该试剂盒适用于心血管疾病动物模型研究、降压药物作用机制探索、内分泌调控实验等科研领域，为小鼠血清或血浆样本中ANG-Ⅱ的动态监测提供可靠工具，实验结果需结合标准曲线进行定量计算。
别名：

angiotensin Ⅱ, angiotensin 2, ANG2
缩写：

ANG-Ⅱ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

15.6 pg/ml-1000 pg/ml.
灵敏度：

7.8 pg/ml.
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-94
1:2	Average %	91
	Range %	85-96
1:4	Average %	90
	Range %	84-98
1:8	Average %	93
	Range %	86-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

1000

0.161

0.166

0.164

500

0.185

0.192

0.189

250

0.228

0.237

0.233

125

0.334

0.324

0.329

62.5

0.568

0.576

0.572

31.25

0.902

0.891

0.897

15.6

1.208

1.217

1.212

2.176

2.223

2.199

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Human placental mesenchymal stem cells transplantation repairs the alveolar epithelial barrier to alleviate lipopolysaccharides-induced acute lung injury W Yu, Y Lv, R Xuan, P Han, H Xu, X Ma,Biochemical Pharmacology,2024
ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway F Chen,Journal of translational medicine,2023
Recombinant human angiotensin-converting enzyme 2 plays a protective role in mice with sepsis-induced cardiac dysfunction through multiple signaling pathways dependent on converting angiotensin II to angiotensin 1–7 C Wu,Annals of Translational Medicine,2023
Bone morphogenetic protein 4 in perivascular adipose tissue ameliorates hypertension through regulation of angiotensinogen WJ Mu,Frontiers in cardiovascular medicine,2022
Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry Zilun L,Cell Metabolism,2022
PSGL1-deficient mice develop spontaneous pulmonary hypertension associated to systemic sclerosis Gonzalez-Tajuelo R, et al,Arthritis & Rheumatology,2019
The combination effects of Shen-Ling-Bai-Zhu on promoting apoptosis of transplanted H22 hepatocellular carcinoma in mice receiving chemotherapy. Xi S.et al,J Ethnopharmacol.,2016
The effects of different angiotensin II type 1 receptor blockers on the regulation of the ACE-AngII-AT1 and ACE2-Ang(1-7)-Mas axes in pressure overload-induced cardiac remodeling in male mice. Wang X.et al,J Mol Cell Cardiol.,2016
Pitavastatin-attenuated cardiac dysfunction in mice with dilated cardiomyopathy via regulation of myocardial calcium handling proteins WEI HU et al,Acta pharmaceutica,2014
Control of Adipogenesis by the Autocrine Interplays between Angiotensin (1-7)/Mas receptor and Angiotensin II/AT1 receptor Signaling Pathways Than A et al,J Biol Chem,2013

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Intra-assay Precision (Precision within an assay): CV%<12%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.