Dog angiotensin Ⅱ (ANG-Ⅱ) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬血管紧张素Ⅱ（ANG-Ⅱ）酶联免疫试剂盒（货号：CSB-E15778c）采用双抗体夹心ELISA技术，专为犬血清或血浆样本中ANG-Ⅱ的定量检测而开发。ANG-Ⅱ作为肾素-血管紧张素系统的核心效应肽，通过调节血管收缩、醛固酮分泌及水盐代谢参与血压稳态和心血管功能研究，是犬类心血管疾病、肾脏病理及内分泌机制研究的重点靶标。本试剂盒检测灵敏度高，线性范围覆盖6.25至400 pg/mL，可精准捕获样本中低浓度目标分子，实验流程兼容常规酶标仪分析。试剂盒包含预包被抗体、检测抗体、标准品及显色底物等核心组分，适用于科研场景下评估药物干预、疾病模型或生理状态对ANG-Ⅱ水平的影响，例如犬类高血压机制探索、肾脏疾病相关生物标志物分析或激素调控研究，为兽医科研及动物模型开发提供可靠数据支持。所有组分均经过严格验证，实验操作全程无需特殊设备，3.5小时内即可完成检测。
别名：

angiotension Ⅱ, angiotensin 2, ANG2
缩写：

ANG-Ⅱ
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog ANG-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	98
	Range %	92-104
1:4	Average %	88
	Range %	86-93
1:8	Average %	89
	Range %	84-96

回收率：

The recovery of dog ANG-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	100	95-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.