Mouse Pro-Nerve growth factor (proNGF) ELISA kit

产品详情

产品描述：

小鼠神经生长因子前体(proNGF)酶联免疫试剂盒（CSB-EQ027721MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的proNGF含量。小鼠神经生长因子前体（proNGF）是神经生长因子（NGF）的前体形式，参与神经元发育、存活和突触形成。其研究机制涉及与受体结合、信号传导以及与疾病如抑郁症和乳腺癌转移的相关性。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于神经生物学基础研究、疾病动物模型评估及药物干预实验中proNGF表达水平的动态监测，尤其可为帕金森模型、脊髓损伤模型等研究提供定量分析工具，为探索神经发育机制及退行性病变提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

proNGF
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

23.5 pg/mL-1500 pg/mL
灵敏度：

5.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse proNGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	102
	Range %	97-106
1:4	Average %	85
	Range %	81-89
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of mouse proNGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-106
EDTA plasma (n=4)	89	84-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.