Human Pro-Nerve growth factor(proNGF)ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人神经生长因子前体(proNGF)酶联免疫试剂盒（CSB-E17311h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的proNGF含量。proNGF 即前神经生长因子，它是神经生长因子的前体形式。相关研究显示，proNGF 可能参与神经系统发育、损伤修复等过程，其异常表达与神经退行性疾病等发病机制有关，在细胞凋亡调控等方面有着重要作用，是神经领域研究的一个重要靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究神经退行性疾病模型中的生物标志物表达、药物干预效果评估或相关信号通路机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

proNGF
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human proNGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-99
1:2	Average %	93
	Range %	89-96
1:4	Average %	90
	Range %	85-96
1:8	Average %	94
	Range %	88-98

回收率：

The recovery of human proNGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.