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  4. Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

Mouse Macrophage Inflammatory Protein 1β,MIP-1β ELISA kit

  • 中文名称：
    小鼠巨噬细胞炎性蛋白1β(MIP-1β/CCL4)酶联免疫试剂盒
  • 货号：
    CSB-E04665m-IS
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠巨噬细胞炎性蛋白1β（MIP-1β/CCL4）酶联免疫检测试剂盒（货号：CSB-E04665m-IS），基于双抗体夹心法原理设计，可对血清、血浆、细胞培养上清及组织匀浆样本中的靶蛋白进行定量检测，灵敏度下限为3.12 pg/mL，检测范围为3.12-200 pg/mL。MIP-1β作为CC趋化因子家族成员，主要由活化T细胞、巨噬细胞等分泌，通过介导炎症细胞趋化参与多种免疫调控过程，其表达水平与感染性疾病、自身免疫病及肿瘤微环境调控等研究密切相关。该试剂盒适用于体外研究炎症反应机制、免疫细胞迁移调控、疾病模型构建等科研场景，可通过标准曲线精确分析样本中MIP-1β的动态浓度变化，为探索趋化因子在免疫病理过程中的作用提供实验支持。试剂盒包含预包被特异性抗体的微孔板及标准品、检测抗体、显色底物等核心组分，实验流程优化后可在常规实验室条件下稳定开展。
  • 别名：
    Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4 ELISA Kit; C-C motif chemokine 4 ELISA Kit; Immune activation protein 2 ELISA Kit; ACT-2 ELISA Kit; ACT2 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Protein H400 ELISA Kit; SIS-gamma ELISA Kit; Small-inducible cytokine A4 ELISA Kit
  • 缩写：
    CCL4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    102

    Range %

    95-108

    1:2

    Average %

    96

    Range %

    91-102

    1:4

    Average %

    91

    Range %

    87-96

    1:8

    Average %

    90

    Range %

    86-96

  • 回收率：

    The recovery of mouse MIP-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    88-98

    EDTA plasma (n=4)

    97

    91-103

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    200

    2.483

    2.634

    2.558

    2.386

    100

    1.530

    1.617

    1.574

    1.401

    50

    1.009

    0.957

    0.983

    0.811

    25

    0.557

    0.529

    0.543

    0.371

    12.5

    0.350

    0.331

    0.340

    0.168

    6.25

    0.244

    0.253

    0.249

    0.076

    3.12

    0.196

    0.184

    0.190

    0.018

    0

    0.177

    0.168

    0.172

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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CCL4 antibodies

CCL4 Proteins

CCL4 ELISA kits

靶点详情

  • 功能：
    Monokine with inflammatory and chemokinetic properties.
  • 基因功能参考文献：
    1. CCL4-CCR5 axis can contribute to breast cancer metastasis to bone by mediating the interaction between cancer cells and fibroblasts in bone cavity. PMID: 27177471
    2. YY1 regulates lung CCL4 transcription in pulmonary tuberculosis. PMID: 26786659
    3. Ccl4 mRNAs increased within 5 h after injury in mouse cortical slices. PMID: 25895671
    4. CCL4 and CCL5 expression was higher in livers of infected WSX-1(-/-) mice than infected WT mice, and hepatic CD4 T cells from WSX-1(-/-) mice expressed higher levels of CCR5 than cells from WT mice PMID: 24244314
    5. CCL4 upregulation was associated with increased Snail expression and downregulation of p53/PTEN in high-grade PIN and prostate cancer PMID: 23878190
    6. These results suggest that MIP-1beta is a novel key mediator, and the peripheral MIP-1beta-CCR5 axis contributes to neuropathic pain. PMID: 22528550
    7. results indicate that MIP-1beta is involved in the recruitment of bone marrow-derived monocyte lineage cells PMID: 21912378
    8. increased responsiveness of murine eosinophils to MIP-1beta is mediated by CCR5 receptor in mice PMID: 12050188
    9. macrophage inflammatory protein-1beta is a hypoxia-induced neutrophil survival factor. PMID: 15630139
    10. MIP-1beta is overexpressed, and VE-cadherin is underexpressed in heart transplant allografts compared with isografts PMID: 15897346
    11. Overproduction of MIP-1beta in chemokine (C-C motif) receptor 5-deficient mice after collagen II-immunization may contribute partially to the occurrence of arthritis PMID: 15967376
    12. The expression and role CXCL12 and CCL4 and their receptors (CXCR4 and CCR5) in regulating thymocyte migration in conjunction with extracellular matrix during acute T. cruzi infection are investigated. PMID: 16637021
    13. ATF3 appears to be part of a control mechanism that limits the amount of CCL4 released by macrophages, preventing excessive inflammation. PMID: 16982098
    14. Antibody neutralization of CCL4 abrogates the ability of T-cells from IL-4-treated NOD mice to transfer protection against type 1 diabetes. PMID: 17327452

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    KEGG: mmu:20303

    STRING: 10090.ENSMUSP00000019074

    UniGene: Mm.244263

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |