Mouse Laminin,LN ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专为定量检测小鼠血清、血浆、细胞培养上清及组织匀浆中的层连蛋白（LN）设计。层连蛋白是细胞外基质的重要组成成分，广泛分布于基底膜中，参与细胞粘附、迁移及分化等生物学过程，其表达水平与组织修复、纤维化疾病及肿瘤微环境调控密切相关。试剂盒检测范围覆盖1.56 ng/mL至100 ng/mL，通过预包被高特异性捕获抗体与标记检测抗体的协同作用实现精准定量分析，实验步骤标准化，配套提供预混试剂和系列浓度标准品，有效提升检测效率。该产品适用于基础科研领域，如探索组织再生机制、纤维化病理模型（如肝/肺纤维化）的分子标志物动态变化，或体外培养细胞分泌LN的功能研究，为相关疾病机制解析及药物干预效果评估提供可靠工具。试剂盒支持多种生物样本类型，满足不同实验场景需求，且操作流程简便，重复性良好，适用于实验室常规检测与高通量筛选。
别名：

N/A
缩写：

LN
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	91
	Range %	88-96
1:8	Average %	101
	Range %	97-105

回收率：

The recovery of mouse LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.