Mouse Immunoglobulin D(IgD) ELISA Kit
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中文名称:小鼠免疫球蛋白D(IgD)酶联免疫试剂盒
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货号:CSB-E15761m
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO小鼠免疫球蛋白D(IgD)定量检测试剂盒,采用双抗体夹心酶联免疫吸附法(ELISA)原理,适用于血清或血浆样本中IgD的精准定量分析。IgD作为B淋巴细胞表面表达的重要抗体类别,在免疫应答初期通过结合抗原参与免疫调节与信号传导,是研究体液免疫机制和B细胞功能的关键指标。试剂盒检测灵敏度为15.6 ng/mL,线性范围覆盖15.6-1000 ng/mL,通过预包被高特异性捕获抗体与标记检测抗体的协同作用,实现目标蛋白的高效识别与信号放大。该产品可应用于免疫学基础研究领域,如评估实验性自身免疫疾病模型中B细胞活化状态、监测疫苗或免疫调节剂干预后的体液免疫动态变化,以及探究感染或炎症过程中IgD介导的免疫调控机制。严格优化的反应体系确保实验重复性,操作流程包含标准品稀释、样本孵育、显色及终止步骤,配套提供完整试剂与操作指南,满足科研场景下对小鼠IgD水平的定量分析需求。
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别名:N/A
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缩写:IgD
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma
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检测范围:15.6 ng/mL-1000 ng/mL
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灵敏度:3.9 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:Intra-assay Precision (Precision within an assay): CV%<8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays): CV%<10%Three samples of known concentration were tested in twenty assays to assess.
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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回收率:
The recovery of mouse IgD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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数据处理:
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货期:3-5 working days
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