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中文名称:人免疫球蛋白D(IgD)酶联免疫试剂盒
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货号:CSB-E15051h
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO人免疫球蛋白D(IgD)酶联免疫检测试剂盒(货号:CSB-E15051h),采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中IgD的浓度,检测灵敏度达0.024 ng/mL,线性范围为0.024-100 ng/mL,可精准覆盖生理及实验条件下IgD的表达水平。IgD作为B细胞受体重要组分,在免疫应答启动和自身免疫性疾病研究中具有重要价值,本试剂盒通过高特异性抗体对实现靶标捕获与信号放大,适用于免疫学基础研究、炎症机制探索或免疫相关疾病模型中IgD动态变化的定量分析。实验操作兼容常规酶标仪检测,提供标准品及预包被板条,支持科研人员高效开展体外样本检测,为免疫调控研究、药物效应评估或生物标志物筛选提供可靠数据支持。
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别名:N/A
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缩写:IgD
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.024 ng/mL-100 ng/mL
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灵敏度:0.024 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IgD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 91 Range % 87-98 1:200 Average % 95 Range % 90-102 1:400 Average % 93 Range % 87-99 1:800 Average % 94 Range % 86-105 -
回收率:
The recovery of human IgD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 85-96 EDTA plasma (n=4) 92 88-96 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.120 2.094 2.107 2.005 25 1.655 1.637 1.646 1.544 6.25 0.995 1.005 1.000 0.898 1.56 0.515 0.507 0.511 0.409 0.39 0.413 0.408 0.411 0.309 0.1 0.204 0.210 0.207 0.105 0.024 0.138 0.132 0.135 0.033 0 0.100 0.103 0.102 -
数据处理:
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货期:3-5 working days
引用文献
- Immunomodulatory constituents of human breast milk and immunity from bronchiolitis Chunyu Li.et al,Italian Journal of Pediatrics,2017
相关产品
靶点详情
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最新研究进展:IgD是一种抗体分子,也是B淋巴细胞表面的一种受体。它在机体的免疫防御中起着重要作用,通过与抗原结合,诱导B细胞的激活和增殖,促进免疫应答的发生。最近的研究表明,IgD在机体的免疫调节中具有重要作用。一项研究发现,在人类B细胞中,IgD能够与TLR7相互作用,从而抑制炎症介质的产生。另一项研究发现,在小鼠模型中,IgD能够抑制胰岛素样生长因子1(IGF1)的信号通路,从而降低肥胖相关的代谢疾病的发生率。
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