Mouse C-telopeptide of type Ⅱ collagen(CTX-Ⅱ) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠Ⅱ型胶原C端肽（CTX-Ⅱ）酶联免疫检测试剂盒（货号CSB-E16211m），专为科研领域设计，适用于定量分析血清、血浆及组织匀浆样本中的CTX-Ⅱ含量。CTX-Ⅱ是Ⅱ型胶原蛋白降解的特异性生物标志物，其水平变化可反映关节软骨代谢状态，在骨关节炎模型研究、软骨退行性病变机制探索及药物干预效果评估中具有重要应用价值。试剂盒采用双抗体夹心法原理，通过预包被高特异性抗体实现精准捕获目标蛋白，检测灵敏度达3.12 pg/mL，线性范围为3.12-200 pg/mL，可覆盖多种实验条件下的浓度梯度需求。该试剂盒操作便捷，支持科研人员高效完成软骨代谢相关疾病的动物模型构建、病理机制分析以及潜在治疗靶点的验证工作，为关节炎、骨质疏松等骨骼系统疾病的分子机制研究提供可靠工具。
别名：

C-telopeptide of type 2 collagen(CTX-2),_x000D_ C-telopeptide of type II collagen(CTX-II)
缩写：

CTX-Ⅱ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	93
	Range %	86-95
1:40	Average %	99
	Range %	96-102
1:80	Average %	92
	Range %	87-95
1:160	Average %	96
	Range %	93-99

回收率：

The recovery of mouse CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	94	91-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.