Rabbit cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于定量检测兔血清、血浆或组织匀浆样本中Ⅱ型胶原C端肽（CTX-Ⅱ）的酶联免疫试剂盒（货号：CSB-E16213Rb），其检测原理为双抗体夹心法，检测灵敏度为0.625 ng/mL，线性范围为0.625-40 ng/mL。CTX-Ⅱ是Ⅱ型胶原降解的产物，作为软骨代谢的重要标志物，其水平变化可反映关节软骨破坏程度，常用于骨关节炎、类风湿性关节炎等疾病模型中软骨退行性病变的机制研究。该试剂盒通过特异性捕获目标蛋白并结合酶标抗体实现精准定量，适用于科研场景中评估实验动物软骨代谢状态、筛选保护软骨组织的候选化合物，或探究病理条件下胶原降解的动态变化。样本兼容性覆盖常规生物液体及组织样本，操作流程标准化，可为体外实验提供稳定的数据支持，助力关节疾病相关的基础研究与药物开发。
别名：

cross linked C-telopeptide of type 2 collagen (CTX-2),_x000D_ cross linked C-telopeptide of type II collagen (CTX-II)
缩写：

CTX-Ⅱ
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-103
1:2	Average %	90
	Range %	86-99
1:4	Average %	95
	Range %	90-102
1:8	Average %	92
	Range %	83-99

回收率：

The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	95	89-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.