Human 5-Hydroxytryptamine,5HT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人5羟色胺（5-HT）酶联免疫检测试剂盒（货号：CSB-E08363h），基于竞争法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中的5-HT浓度。5-羟色胺作为一种重要的单胺类神经递质，广泛参与情绪调节、睡眠周期和胃肠功能等生理过程，其水平变化与神经精神疾病、代谢综合征等研究密切相关。CUSABIO试剂盒采用特异性抗体包被微孔板，样本中的5-HT分子与酶标记物竞争结合抗体位点，通过显色反应强度计算目标物浓度，检测灵敏度为4 ng/mL，线性范围可达800 ng/mL，满足不同浓度样本的检测需求。该试剂盒特别适用于神经递质代谢研究、精神疾病动物模型分析、药理学实验中的药效评估等科研领域，例如探究抑郁症或肠易激综合征模型中5-HT的动态变化，或筛选影响5-HT合成/代谢的化合物。试剂盒提供预包被板条及标准化试剂，操作流程优化至3小时内完成，兼容常规酶标仪检测，为神经科学、药理学及基础医学研究提供高效可靠的工具。
缩写：

5HT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4 ng/mL-800 ng/mL
灵敏度：

2 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	85
	Range %	80-89
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	89	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.