Human Hydroxyacid oxidase 1(HAO1) ELISA kit

中文名称：

人羟基酸氧化酶1(HAO1)酶联免疫试剂盒
货号：

CSB-EL010127HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人羟基酸氧化酶1(HAO1)酶联免疫试剂盒（CSB-EL010127HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HAO1含量。HAO1，即羟基酸氧化酶1，是一种FMN依赖的α-羟基酸脱氢酶家族成员，主要在肝脏的过氧化物酶体中氧化甘醇酸生成甘醇酸内酯，同时产生过氧化氢。其表达在氧化应激期间可能下调，以防止过氧化氢在过氧化物酶体中过量生成，可能是一种对氧化损伤的响应机制。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于科研场景中HAO1的功能研究，如代谢性疾病模型构建、肝肾组织病理分析、药物作用靶点筛选等方向，为探索代谢调控机制及疾病干预策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

(S) 2 hydroxy acid oxidase ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit; GOX1 ELISA Kit; HAO1 ELISA Kit; HAOX1 ELISA Kit; HAOX1_HUMAN ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; MGC142225 ELISA Kit; MGC142227 ELISA Kit; OTTHUMP00000030231 ELISA Kit
缩写：

HAO1
Uniprot No.：

Q9UJM8
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.69 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	98
	Range %	93-112
1:10	Average %	107
	Range %	103-109
1:20	Average %	93
	Range %	90-97
1:40	Average %	88
	Range %	86-94

回收率：

The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	92	90-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.193	2.369	2.281	2.097
600	1.700	1.710	1.705	1.521
300	1.253	1.262	1.258	1.074
150	0.878	0.898	0.888	0.704
75	0.532	0.566	0.549	0.365
37.5	0.411	0.422	0.417	0.233
18.75	0.231	0.222	0.227	0.043
0	0.177	0.190	0.184

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
基因功能参考文献：
1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
亚细胞定位：

Peroxisome.
蛋白家族：

FMN-dependent alpha-hydroxy acid dehydrogenase family
组织特异性：

Liver.
数据库链接：

HGNC: 4809

OMIM: 605023

KEGG: hsa:54363

STRING: 9606.ENSP00000368066

UniGene: Hs.193640

Human Hydroxyacid oxidase 1(HAO1) ELISA kit

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