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  4. Human Epidermal growth factor-like protein 6(EGFL6) ELISA kit

Human Epidermal growth factor-like protein 6(EGFL6) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    人表皮生长因子样蛋白6(EGFL6)酶联免疫试剂盒
  • 货号：
    CSB-EL007475HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人表皮生长因子样蛋白6（EGFL6）酶联免疫检测试剂盒（货号：CSB-EL007475HU），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的EGFL6蛋白水平。EGFL6是表皮生长因子家族成员，主要参与血管生成、组织修复等生理过程，近年研究表明其在肿瘤微环境调控中可能发挥重要作用，是癌症机制研究的潜在靶标。本试剂盒检测灵敏度高，线性范围为78 pg/mL至5000 pg/mL，可精准覆盖生理及病理条件下EGFL6的表达量变化，操作步骤严格优化，具备良好的重复性和稳定性。适用于体外研究EGFL6在肿瘤发生发展、血管生成相关疾病中的表达模式，也可用于细胞培养上清或组织样本的蛋白动态监测，为探索其分子机制提供可靠工具。本产品仅用于科学研究，实验全程无需特殊设备，配套提供标准品、预包被板及显色试剂，3.5小时内即可完成检测。
  • 别名：
    EGF like domain multiple 6 ELISA Kit; EGF repeat containing protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; EGF-like-domain multiple 6 ELISA Kit; egfl6 ELISA Kit; EGFL6_HUMAN ELISA Kit; epidermal growth factor like protein 6 ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; MAEG ELISA Kit; MAM and EGF domain containing ELISA Kit; MAM and EGF domains containing gene protein ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; W80 ELISA Kit
  • 缩写：
    EGFL6
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 104
    Range % 97-108
    1:2 Average % 101
    Range % 93-105
    1:4 Average % 97
    Range % 89-101
    1:8 Average % 104
    Range % 94-109
  • 回收率：
    The recovery of human EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 83-94
    EDTA plasma (n=4) 102 95-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    5000 1.972 1.918 1.945 1.800
    2500 1.400 1.439 1.420 1.275
    1250 1.072 1.025 1.049 0.904
    625 0.762 0.734 0.748 0.603
    312 0.521 0.529 0.525 0.380
    156 0.348 0.357 0.353 0.208
    78 0.271 0.283 0.277 0.132
    0 0.148 0.142 0.145 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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EGFL6 antibodies

EGFL6 Proteins

靶点详情

  • 功能：
    May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
  • 基因功能参考文献：
    1. In conclusion, our results suggest that plasma level of EGFL6 may be useful to assess disease progression, and especially advanced T status and higher TNM stage in patients with oral squamous cell carcinoma. PMID: 28539817
    2. EGFL6 is an angiocrine and tumor cell factor that promotes tumorigenesis via SHP2-mediated signaling. EGFL6 stimulates ALDH+ ovarian cancer stem cell to migrate and undergo asymmetric division. PMID: 27803106
    3. EGFL6 was overexpressed in benign meningioma tissues and serum. PMID: 23285163
    4. results indicate that human EGFL6 is a paracrine/autocrine growth factor of adipose tissue up-regulated in obesity and potentially involved in the process of adipose tissue expansion and the development of obesity PMID: 20574786
    5. There is an absence of GNAS and EGFL6 mutations in common human cancers. PMID: 18038328

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix, basement membrane.
  • 蛋白家族：
    Nephronectin family
  • 数据库链接：

    HGNC: 3235

    OMIM: 300239

    KEGG: hsa:25975

    STRING: 9606.ENSP00000369976

    UniGene: Hs.12844

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