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  4. Human Cystatin-F(CST7)ELISA kit

Human Cystatin-F(CST7)ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    人胱抑素F(CST7)酶联免疫试剂盒
  • 货号：
    CSB-E17504h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    人胱抑素F(CST7)酶联免疫试剂盒（CSB-E17504h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的CST7含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研场景中CST7的功能研究，例如探究其在炎症反应、肿瘤侵袭或神经病变模型中的表达变化规律，或通过体外细胞培养实验分析特定刺激下CST7的分泌动态，为疾病机制研究及潜在生物标志物探索提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CMAP ELISA Kit; CST 7 ELISA Kit; Cst7 ELISA Kit; Cystatin 7 ELISA Kit; cystatin F (leukocystatin) ELISA Kit; Cystatin like metastasis associated protein ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-F ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; Cystatin7 ELISA Kit; CystatinF ELISA Kit; CYTF_HUMAN ELISA Kit; dJ568C11.1 ELISA Kit; Leukocystatin ELISA Kit
  • 缩写：
    CST7
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 85-97
    1:2 Average % 98
    Range % 91-101
    1:4 Average % 94
    Range % 85-97
    1:8 Average % 90
    Range % 84-95
  • 回收率：
    The recovery of human CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 94-107
    EDTA plasma (n=4) 106 98-112
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.319 2.235 2.277 2.124
    20 1.685 1.671 1.678 1.525
    10 0.978 0.927 0.953 0.800
    5 0.641 0.627 0.634 0.481
    2.5 0.355 0.369 0.362 0.209
    1.25 0.247 0.260 0.254 0.101
    0.625 0.195 0.188 0.192 0.039
    0 0.151 0.155 0.153 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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CST7 Proteins

相关问答

■ 常见问题解答
Q:

Does your kit # CSB-E17504h or any of its components contain recombinant proteins?
If so, what is the expression system of these recombinants?

A:
Thanks for your inquiry!
CSB-E17504h contains recombinant proteins.
Standard is 293 cell-derived recombinant protein.
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靶点详情

  • 功能：
    Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
  • 基因功能参考文献：
    1. during monocyte to macrophage differentiation, the endosomal/lysosomal proteolytic activity can be regulated by cystatin F whose expression is under the control of transcriptional factor C/EBP alpha. PMID: 30033148
    2. Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells. PMID: 22365146
    3. Transgenic cystatin F is expressed in model mice only during demyelination; in chronic demyelination, remyelinating ability is lost. PMID: 21344476
    4. cystatin F is an intracellular cysteine peptidase inhibitor with readily regulated expression PMID: 12423348
    5. CST7 is secreted, but artificial modification of its C-terminus can induce its endocytic targeting. PMID: 15212960
    6. Cystatin F was not colocalized with cystatin C shich suggests distinct functions for these two cysteine protease inhibitors in U937 cells. PMID: 15752368
    7. analysis of reduction-dependent activation of human cystatin F PMID: 16601115
    8. A latent protease inhibitor which is itself regulated by proteolysis in the endocytic pathway. PMID: 18256700
    9. Data demonstrate the addition of N-linked sugars to an Asn-X-Cys motif in cystatin F and suggest that the mannose 6-phosphate sorting machinery is used to divert cystatin F from the secretory pathway and to mediate its uptake from extracellular pools. PMID: 19192250

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  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 蛋白家族：
    Cystatin family
  • 组织特异性：
    Primarily expressed in peripheral blood cells and spleen.
  • 数据库链接：

    HGNC: 2479

    OMIM: 603253

    KEGG: hsa:8530

    STRING: 9606.ENSP00000420384

    UniGene: Hs.143212

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |