Human Cortisol ELISA Kit

• A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
• Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
• Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
• Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
• HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
• HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
• Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
• TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
• Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells)
• An Instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

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