Human Chordin-like protein 1(CHRDL1) ELISA kit

Human Chordin-like protein 1(CHRDL1) ELISA kit

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中文名称：

人腱蛋白样蛋白1(CHRDL1)酶联免疫试剂盒
货号：

CSB-EL005378HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人腱蛋白样蛋白1(CHRDL1)酶联免疫试剂盒（CSB-EL005378HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CHRDL1含量。人腱蛋白样蛋白1（CHRDL1）是一种与BMP4结合的拮抗剂，能阻断其受体相互作用，将神经干细胞从胶质生成重定向到神经发生，支持大脑中的神经元分化。研究显示，CHRDL1在背腹轴形成、胚胎骨发育和视网膜血管生成中发挥重要作用，并通过调节BMP4的作用来调节视网膜血管生成。试剂盒检测范围为0.156 ng/mL-10 ng/mL，支持科研人员开展CHRDL1在神经生物学、骨骼发育、肿瘤微环境调控等领域的机制研究，尤其适用于探究CHRDL1在疾病模型中的动态表达及其与BMP通路互作的分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CHL ELISA Kit; chordin like 1 ELISA Kit; Chordin-like protein 1 ELISA Kit; CHRDL1 ELISA Kit; CRDL1_HUMAN ELISA Kit; dA141H5.1 ELISA Kit; neuralin 1 ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; NRLN1 ELISA Kit; Ventroptin ELISA Kit; VOPT ELISA Kit
缩写：

CHRDL1
Uniprot No.：

Q9BU40
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-93
1:2	Average %	104
	Range %	98-107
1:4	Average %	96
	Range %	88-99
1:8	Average %	96
	Range %	91-99

回收率：

The recovery of human CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	99	94-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.551	2.487	2.519	2.354
5	2.164	2.112	2.138	1.973
2.5	1.651	1.688	1.670	1.505
1.25	1.043	1.032	1.038	0.873
0.625	0.660	0.645	0.653	0.488
0.312	0.474	0.467	0.471	0.306
0.156	0.268	0.272	0.270	0.105
0	0.163	0.167	0.165

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.
基因功能参考文献：
1. Finally, in vivo experiments confirmed that CHRDL1 acted as a tumor suppressor gene in suppressing tumor growth and metastasis PMID: 28423564
2. Results show that Chrdl1 expression is upregulated in numerous breast cancer cells following TGF-beta stimulation and demonstrated that Chrdl1 acts as an inhibitor of BMP4-induced migration and invasion. PMID: 26976638
3. These studies have identified and validated new factors enriched in juvenile chondrocytes as compared to adult chondrocytes including secreted extracellular matrix factors chordin-like 1 (CHRDL1) and microfibrillar-associated protein 4 (MFAP4). PMID: 26955889
4. We provide the initial confirmation that X-linked megalocornea is associated with mutations in the CHRDL1 gene. PMID: 24073597
5. CHRDL1 plays a key role in cornea homeostasis as evidenced by disease causing mutations in X-linked megalocornea. PMID: 25712132
6. The detection of mutations in the CHRDL1 gene is useful for differential diagnosis with different forms of megalocornea. PMID: 26020825
7. Novel CHRDL1 mutations in ten families with X-linked megalocornea, are reported. PMID: 25093588
8. CHRDL1 is expressed in the developing human cornea and anterior segment in addition to the retina. PMID: 22284829
9. Hypoxia-induced upregulation of CHL-1 alters the homeostatic balance between BMP-4 and VEGF to synergize with VEGF in driving retinal angiogenesis. PMID: 18587495
10. reports the cloning of chick ventroptin and its importance in topographic retinotectal projection PMID: 11441185
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相关疾病：

Megalocornea 1, X-linked (MGC1)
亚细胞定位：

Secreted.
组织特异性：

Expressed in the developing cornea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex. Expressed in retinal pericytes.
数据库链接：

HGNC: 29861

OMIM: 300350

KEGG: hsa:91851

STRING: 9606.ENSP00000361112

UniGene: Hs.496587

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.