Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

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中文名称：

人羧基肽酶N亚基2(CPN2)酶联免疫试剂盒
货号：

CSB-EL005899HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人羧基肽酶N亚基2(CPN2)酶联免疫试剂盒（CSB-EL005899HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPN2含量。人羧基肽酶N亚基2（CPN2）是一种羧肽酶，参与蛋白质降解和细胞信号传导。其研究机制涉及CPN2在蛋白质降解过程中的作用，以及如何通过调节细胞内信号通路影响细胞功能。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中探索CPN2在心血管疾病、免疫调节或炎症机制中的生物学功能，亦可用于体外模型中蛋白表达水平的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CPN2 ELISA kit; ACBPCarboxypeptidase N subunit 2 ELISA kit; Carboxypeptidase N 83 kDa chain ELISA kit; Carboxypeptidase N large subunit ELISA kit; Carboxypeptidase N polypeptide 2 ELISA kit; Carboxypeptidase N regulatory subunit ELISA kit
缩写：

CPN2
Uniprot No.：

P22792
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CPN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-105
1:2	Average %	106
	Range %	102-112
1:4	Average %	97
	Range %	91-103
1:8	Average %	93
	Range %	89-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.858

2.934

2.896

2.755

2.131

2.167

2.149

2.008

2.5

1.275

1.328

1.302

1.160

1.25

0.837

0.829

0.833

0.692

0.625

0.492

0.521

0.506

0.365

0.312

0.317

0.323

0.320

0.179

0.156

0.238

0.244

0.241

0.100

0.137

0.145

0.141

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
基因功能参考文献：
1. Real-time PCR and western blotting show the following CPN2 expression: minimal in MDA-MB-468 cells but obvious in MDA-MB-231 and MCF7 cells. MDA-MB-231 breast cancer cell lines were selected for the control group and CPN2 was knocked out for the experimental group. PMID: 26860443
亚细胞定位：

Secreted.
数据库链接：

HGNC: 2313

OMIM: 603104

KEGG: hsa:1370

STRING: 9606.ENSP00000319464

UniGene: Hs.528368

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.