Human Acyloxyacyl hydrolase(AOAH) ELISA kit

中文名称：

人酰基羧酸水解酶AOAH）酶联免疫试剂盒
货号：

CSB-EL001853HU
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

人酰基羧酸水解酶AOAH）酶联免疫试剂盒（CSB-EL001853HU）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的AOAH含量。AOAH（酰基羧酸水解酶）是一种在多种细胞中表达的宿主脂肪酶，能够选择性地去除革兰氏阴性菌LPS脂质A的二级酰基链，从而灭活LPS，减轻炎症和组织损伤。研究表明，AOAH在感染性疾病中发挥重要作用，同时也在过敏性哮喘、银屑病等自身免疫性疾病中扮演重要角色。研究机制显示，AOAH通过降解LPS，调节免疫反应，减轻炎症。试剂盒检测范围为0.156 ng/mL-10 ng/mL，其应用场景包括但不限于脂质代谢异常研究、炎症信号通路解析、免疫细胞功能评估等基础科研领域，例如在动脉粥样硬化模型、肝脏疾病或肠道菌群相关免疫调控实验中定量追踪AOHA的表达动态变化，为揭示其在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Acyloxyacyl hydrolase (neutrophil) ELISA Kit; Acyloxyacyl hydrolase large subunit ELISA Kit; AOAH ELISA Kit; AOAH_HUMAN ELISA Kit
缩写：

AOAH
Uniprot No.：

P28039
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AOAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-96
1:2	Average %	97
	Range %	91-103
1:4	Average %	99
	Range %	82-106
1:8	Average %	87
	Range %	81-92

回收率：

The recovery of human AOAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	101	94-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.680	2.769	2.725	2.620
5	2.246	2.124	2.185	2.080
2.5	1.495	1.387	1.441	1.336
1.25	0.820	0.837	0.829	0.724
0.625	0.475	0.486	0.481	0.376
0.312	0.281	0.272	0.277	0.172
0.156	0.204	0.210	0.207	0.102
0	0.104	0.105	0.105

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.
基因功能参考文献：
1. analysis of AOAH function through its crystal structure and complex with lipopolysaccharide PMID: 29343645
2. AOAH - Gene involved in innate immunity that have been associated with Chronic Rhinosinusitis. PMID: 27888910
3. AOAH rs60023210 variants may be associated with carotid intima-media thickness at bifurcation. PMID: 26319989
4. Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population. PMID: 22723975
亚细胞定位：

Secreted. Cytoplasmic vesicle.
数据库链接：

HGNC: 548

OMIM: 102593

KEGG: hsa:313

STRING: 9606.ENSP00000258749

UniGene: Hs.488007

Human Acyloxyacyl hydrolase(AOAH) ELISA kit

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