Hamster Interleukin 6,IL-6 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专为定量检测仓鼠样本中白介素6（IL-6）设计。IL-6是一种多功能细胞因子，在炎症反应、免疫调节及造血系统中发挥重要作用，其浓度变化常与感染性疾病、自身免疫病理过程及肿瘤微环境调控相关。本产品适用于血清、血浆、细胞培养上清液及组织匀浆四类样本检测，灵敏度达7.8 pg/mL，线性检测范围覆盖7.8-500 pg/mL，可精准反映微量IL-6水平变化。试剂盒包含预包被抗体的96孔微孔板、生物素标记检测抗体、系列浓度标准品及配套显色试剂，通过链霉亲和素-HRP系统实现信号放大，操作流程包含4小时孵育步骤。科研人员可将其应用于炎症机制研究、免疫治疗模型评估或药物研发中的细胞因子动态监测，特别适合体外实验环境下的定量分析需求。所有组分需在2-8℃避光保存，有效期为12个月。本产品严格限定于科学研究用途，实验全程无需特殊设备，兼容常规酶标仪进行450nm波长检测。
缩写：

IL-6
种属：

Mesocricetus auratus (Golden hamster)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	95
	Range %	91-99
1:4	Average %	96
	Range %	91-100
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of hamster IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	91	85-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.